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首页> 外文OA文献 >The Hypothetical Protein CT813 Is Localized in the Chlamydia trachomatis Inclusion Membrane and Is Immunogenic in Women Urogenitally Infected with C. trachomatis
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The Hypothetical Protein CT813 Is Localized in the Chlamydia trachomatis Inclusion Membrane and Is Immunogenic in Women Urogenitally Infected with C. trachomatis

机译：假设蛋白CT813定位于沙眼衣原体包膜中，在泌尿生殖道沙眼衣原体感染的女性中具有免疫原性。

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Using antibodies raised with chlamydial fusion proteins, we have localized a protein encoded by hypothetical open reading frame CT813 in the inclusion membrane of Chlamydia trachomatis. The detection of the C. trachomatis inclusion membrane by an anti-CT813 antibody was blocked by the CT813 protein but not unrelated fusion proteins. The CT813 protein was detected as early as 12 h after chlamydial infection and was present in the inclusion membrane during the entire growth cycle. All tested serovars from C. trachomatis but not other chlamydial species expressed the CT813 protein. Exogenously expressed CT813 protein in HeLa cells displayed a cytoskeleton-like structure similar to but not overlapping with host cell intermediate filaments, suggesting that the CT813 protein is able to either polymerize or associate with host cell cytoskeletal structures. Finally, women with C. trachomatis urogenital infection developed high titers of antibodies to the CT813 protein, demonstrating that the CT813 protein is not only expressed but also immunogenic during chlamydial infection in humans. In all, the CT813 protein is an inclusion membrane protein unique to C. trachomatis species and has the potential to interact with host cells and induce host immune responses during natural infection. Thus, the CT813 protein may represent an important candidate for understanding C. trachomatis pathogenesis and developing intervention and prevention strategies for controlling C. trachomatis infection.
机译：使用衣原体融合蛋白产生的抗体，我们将由假设的开放阅读框CT813编码的蛋白定位在沙眼衣原体的包涵膜中。抗CT813抗体可检测沙眼衣原体包涵膜，但CT813蛋白可阻断该检测，但不相关的融合蛋白可阻止该检测。早在衣原体感染后12小时就检测到了CT813蛋白，并在整个生长周期中均存在于包涵膜中。来自沙眼衣原体的所有测试血清均表达CT813蛋白，但其他衣原体物种均未表达。 HeLa细胞中外源表达的CT813蛋白表现出类似于细胞骨架的结构，但与宿主细胞中间丝不重叠，这表明CT813蛋白能够聚合或与宿主细胞的细胞骨架结合。最后，患有沙眼衣原体泌尿生殖道感染的妇女产生了高滴度的CT813蛋白抗体，表明CT813蛋白不仅在人衣原体感染期间表达，而且具有免疫原性。总之，CT813蛋白是沙眼衣原体物种特有的包膜蛋白，在自然感染过程中具有与宿主细胞相互作用并诱导宿主免疫应答的潜力。因此，CT813蛋白可能代表理解沙眼衣原体的发病机理和开发控制沙眼衣原体感染的干预和预防策略的重要候选人。

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